AbbVie is seeking a driven and innovative Associate Scientist II, In Vitro Pharmacology, to join the mRNA-tLNP group. The Associate Scientist II, In Vitro Pharmacology, will develop and perform cell-based assays to generate data that advance mRNA-tLNP pipeline programs from early discovery through IND-enabling studies, with an emphasis on applications in oncology and autoimmune diseases.

Responsibilities

Execution in vitro assays to evaluate mRNA-tLNP-mediated T-cell reprogramming, including assessment of cytotoxicity, T-cell effector function, and immune cell phenotyping.
Isolate PBMCs and T cells from leukopaks.
Use aseptic techniques to culture mammalian cells, including tumor cell lines, T cells, and PBMCs.
Make stable tumor cell lines using techniques such as electroporation, viral delivery, and nanoparticle transfections.
Perform routine tasks competently and independently, and generate reliable and consistent results.
Demonstrate skill in multiple techniques and appropriately modify protocols, as required, to suit the changing needs of research or development sciences.
Maintain and implement good laboratory practice including an accurate online laboratory notebook system, instrument and reagent validation, and personal safety precautions.
Work collaboratively in a cross-functional team environment. Organize and communicate data (written and oral) and participate in project team meetings.

Qualifications

Bachelor’s Degree, or equivalent education, with typically 3 or more years’ experience or Master’s Degree or equivalent education (no additional experience), in immunology, cell and molecular biology or related field.
Previous experience in chimeric antigen receptor (CAR) production and evaluation is desirable.
Experience with aseptic/sterile techniques culturing of mammalian cells, including cell lines, T cells, and PBMCs.
Experience with viral and non-viral gene transfer techniques to engineer mammalian cells.
Experience designing and executing assays to assess T cell function including cytotoxicity, activation, proliferation and cytokine profiling.
Proficiency in flow cytometry and data processing.
Experience designing and executing plate-based assays, including ELISA and Luminex/MSD.
Experience with scientific data analysis software including CellEngine and Tableau is highly preferred.
Experience with digital PCR is desirable.

Additional Information

Applicable only to applicants applying to a position in any location with pay disclosure requirements under state or local law:

The compensation range described below is the range of possible base pay compensation that the Company believes in good faith it will pay for this role at the time of this posting based on the job grade for this position. Individual compensation paid within this range will depend on many factors including geographic location, and we may ultimately pay more or less than the posted range. This range may be modified in the future.
We offer a comprehensive package of benefits including paid time off (vacation, holidays, sick), medical/dental/vision insurance and 401(k) to eligible employees.
This job is eligible to participate in our short-term incentive programs.

Note: No amount of pay is considered to be wages or compensation until such amount is earned, vested, and determinable. The amount and availability of any bonus, commission, incentive, benefits, or any other form of compensation and benefits that are allocable to a particular employee remains in the Company's sole and absolute discretion unless and until paid and may be modified at the Company’s sole and absolute discretion, consistent with applicable law.

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